🧪 Organ Bath: A Complete Guid
📌 Introduction An organ bath is an experimental setup used in pharmacology and physiology laboratories. It is mainly used to study the effect of different drugs and chemicals on isolated tissues or organs (like ileum, uterus, trachea, heart). This method helps scientists understand how drugs act on smooth muscles and helps in drug discovery, testing, and education. ⚙️ Components of Organ Bath (Well-Labeled) A standard organ bath contains the following parts: Tissue Chamber – A glass container filled with a nutrient solution (like Tyrode’s solution) where the isolated tissue is placed. Aerator – Supplies oxygen (O₂) and carbon dioxide (CO₂) mixture to keep the tissue alive and functional. Heating Coil/Water Jacket – Maintains the temperature (around 37°C for mammalian tissues). Lever/Transducer System – Records the contraction and relaxation of the tissue. Kymograph/Recorder – Earlier, a smoked drum was used; now, digital recorders are used to display gr...